منابع مشابه
Detection of Functional Matrix Metalloproteinases by Zymography
Matrix metalloproteinases (MMPs) are zinc-containing endopeptidases. They degrade proteins by cleavage of peptide bonds. More than twenty MMPs have been identified and are separated into six groups based on their structure and substrate specificity (collagenases, gelatinases, membrane type [MT-MMP], stromelysins, matrilysins, and others). MMPs play a critical role in cell invasion, cartilage de...
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In situ gelatin zymography is a simple technique providing valuable information about the cellular and tissue localization of gelatinases. Until recently, the use of this technique has been confined to soft, relatively homogeneous tissue. In this report in situ zymography has been utilized to assess the sub-lamellar location of gelatinases in the hard, semi-keratinized epidermal layer and the a...
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Current evidence regarding the regulation of urokinase-dependent ex tracellular proteolysis indicates that plasminogen activation is a surface associated process. We have compared the histological localization of urokinase plasminogen activator (uPA) and urokinase plasminogen acti vator receptor (uPAR) in breast cancer sections using a panel of mono clonal antibodies. First, the ability of six ...
متن کاملDetection of femtomole quantities of mature cathepsin K with zymography.
Cathepsin K, the most potent mammalian collagenase, has been implicated in osteoporosis, cancer metastasis, atherosclerosis, and arthritis. Although procathepsin K is stable and readily detected, the active mature cathepsin K eludes detection by in vitro methods due to its shorter half-life and inactivation at neutral pH. We describe, for the first time, reliable detection, visualization, and q...
متن کاملZymography of monophenolase and o-diphenolase activities of polyphenol oxidase.
Polyphenol oxidase (PPO) (monophenol, o-diphenol: oxygen oxidoreductase; EC 1.14.18.1) is a binuclear copper-cluster-containing monooxygenase ubiquitously present in biological systems (1, 2). PPO may be distinguished from the related oxidase laccase [p-diphenol:oxygen oxidoreductase; EC 1.10.3.2] by its substrate specificity and the type and number of catalytic coppers. PPO catalyzes the oxida...
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ژورنال
عنوان ژورنال: Japanese Journal of Thrombosis and Hemostasis
سال: 1994
ISSN: 1880-8808,0915-7441
DOI: 10.2491/jjsth.5.121